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KMID : 0613820200300100851
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Journal of Life Science
2020 Volume.30 No. 10 p.851 ~ p.859
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The Effect of Saururus Chinensis Extracts on Antioxidant Activity and Melanin Synthesis
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Jung So-Mi
Park Hye-Jeong
Kim Jae-Ho
Oh Yung-Hee
Kim Moon-Moo
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Abstract
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Saururus chinensis has white roots, leaves, and flowers and is known to have antibacterial activity and anti-cancer efficacy. The aim of this study was to investigate the effect of the ethyl acetate fraction of a methanol extract of Saururus chinensis (SCEA) on antioxidant activity and melanin synthesis. SCEA at 64 μg/ml showed 62% of the DPPH radical scavenging activity of vitamin C, and its reducing power was 33% greater than that of vitamin C. Tyrosinase activity was 26% higher and melanin synthesis was 44% higher in the presence of SCEA at 64 μg/ml than in a blank group in a dopa oxidation assay. MTT assay showed that SCEA displayed cytotoxicity above 0.5 μg/ml, and SCEA at 1 μg/ml increased melanin synthesis by 69% in live B16F1 cells. SCEA was also separated into 13 fractions by silica column chromatography, and fraction 2 (Fr. 2) showed the highest DPPH radical scavenging activity, reducing power, and melanin synthesis. SCEA also promoted melanin production in live cells. LC-MASS analysis showed that Fr.2 had a molecular weight of 239, and these findings suggest that SCEA could be available for the promotion of melanin synthesis in black hair.
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KEYWORD
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B16F1, melanin synthesis, Saururus chinensis, tyrosinase
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